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(Wireless-NewsWire.Com, June 12, 2018 ) The next generation sequencing market is estimated to represent a global market of USD 5,618 million by 2017 with growth rate of 9.1%. Next-generation sequencing (NGS) involves an advanced level of genetic sequencing which is a level above the conventional methods such as capillary electrophoresis-based Sanger sequencing. NGS procedure is a multi-step process where a number of smaller laboratory processes combine to form the whole next-generation sequencing process. This includes pre-sequencing steps of sample preparation, target enrichment, library preparation under NGS pre-sequencing, the sequencing procedure under NGS Sequencing and data warehousing and data interpretation under NGS data analysis in the workflow outlook.
NGS technology is used for the wide array of sequencing approaches such as transcriptome, genome, and epigenome of any organism. Sequencing techniques differ primarily based on the development of DNA/RNA samples, i.e. tissue type, organism, experimental conditions, and others. This technology is very effective and flexible for the variety of study in large genomic centers to small-scale laboratories. NGS sequencing method is different from Sanger sequencing at a fundamental level in which instead of one fragment being expanded a time, over millions of fragments are expanded in simultaneous reactions which greatly enhance the sequencing capabilities.
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Technology Takeaway
In terms of technological level, next-generation sequencing is categories into Whole Exon Sequencing (WES) , Whole Genome Sequencing (WGS) , Targeted Sequencing and Resequencing Technique. These approaches encompass smaller fragment length sequencing approaches such as de novo sequencing and others. NGS resequencing and de novo sequencing provide laboratories with a capability to produce high throughput sequencing results.
Targeted sequencing captured the significant share of the global market. This sequencing enables researchers to isolate and the sequence-specific subset of genes of the genome. It allows researchers to focus data analysis and specific area of interest time with higher coverage level. For example, targeted resequencing method can cover more than 500-1000× region of genome compared to the traditional WGS study (achieves coverage levels of 30-50× per genome) . This advantage help researcher to identify rare variations which are impossible with WGS and CE-based sequencing techniques.
Application Takeaway
Oncology, Reproductive Health, Clinical Investigation, Consumer Genomics, Immune Monitoring, and others are categorized under application segment. NGS technique has had the greatest applications for clinical research and development of cancer diagnostics and therapeutics. NGS has major applications in oncology wherein it is used to sequence and Catalogue gene mutations for further development of novel cancer diagnostic and therapeutic methodologies. With the expected reduction in the cost of whole-genome sequencing, applications of NGS in oncology will witness the significant increase in demand throughout the forecast period.
SCOPE OF THE REPORT
The scope of this report covers the market by its major segments, which include as follows:
MARKET, BY TECHNOLOGY
Whole Exon Sequencing (WES)
Whole Genome Sequencing (WGS)
Targeted Sequencing
Resequencing Technique
MARKET, BY APPLICATION
Oncology
Reproductive Health
Clinical Investigation
Consumer Genomics
Immune Monitoring
Others
MARKET, BY PRODUCT & SERVICES
Pre-Sequencing Products
Sequencing Services
Data Analysis
MARKET, BY END USE
Academic Research
Hospital Settings
Clinical Laboratories
Others
MARKET, BY REGION
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Key Vendor Takeaway
Companies such as Illumina, Inc., Qiagen, Agilent Technologies, Genomatix GmbH, and Roche are profiles in the study. Wide product offering coupled with increasing research and development activities helped companies to achieve significant revenue share. For example, Illumina offers the wide range of NGS instruments, ranging from the benchtop MiniSeq System to the advanced NovaSeq 6000 System. The company presents comprehensive solutions for NGS workflow such as sequencing instrument, library preparation kits, bioinformatics and automated data analysis tools.
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